Journal: Journal of Oral Microbiology

Article Title: The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis

doi: 10.1080/20002297.2025.2487944

Figure Lengend Snippet: Strains used in this study.

Article Snippet: We compared the alkaline resistance of these six strains, along with two standard laboratory E. faecalis strains (ATCC 29212 and ATCC 51299), through alkaline resistance analysis.

Techniques: Preserving, Over Expression

Journal: Journal of Oral Microbiology

Article Title: The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis

doi: 10.1080/20002297.2025.2487944

Figure Lengend Snippet: Biological characteristics, growth kinetic assay and morphology of E. faecalis at pH 7 and 10. (a) Bacterial suspensions of ATCC 29212, Δ mptD and + mptD . (b, c) crystal violet staining biofilms and biofilm mass analysis of ATCC 29212, Δ mptD and + mptD . (d) Cell morphologies of ATCC 29212, Δ mptD and + mptD at exponential growth phase (10,000×). (e) Growth kinetics of ATCC 29212, Δ mptD and + mptD for 24 h. * p < 0.05; *** p < 0.001; **** p < 0.0001.

Article Snippet: We compared the alkaline resistance of these six strains, along with two standard laboratory E. faecalis strains (ATCC 29212 and ATCC 51299), through alkaline resistance analysis.

Techniques: Kinetic Assay, Staining

Journal: Journal of Oral Microbiology

Article Title: The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis

doi: 10.1080/20002297.2025.2487944

Figure Lengend Snippet: The membrane potential of E. faecalis at pH 7 and 10. (a) Flow cytometry dot plots showing membrane potential of ATCC 29212, Δ mptD and + mptD , gates indicate the proportion of the hyperpolarized cell population. (b, c) membrane potential and permeability of ATCC 29212, Δ mptD and + mptD . **** p < 0.0001.

Article Snippet: We compared the alkaline resistance of these six strains, along with two standard laboratory E. faecalis strains (ATCC 29212 and ATCC 51299), through alkaline resistance analysis.

Techniques: Membrane, Flow Cytometry, Permeability

Journal: Journal of Oral Microbiology

Article Title: The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis

doi: 10.1080/20002297.2025.2487944

Figure Lengend Snippet: The intracellular potassium ions concentration and pH, as well as cellular energy metabolism of E. faecalis at pH 7 and 10. (a) Intracellular potassium ion (K + ) concentration of ATCC 29212, Δ mptD and + mptD are indicated by fluorescence intensity (green). (b) Quantification of intracellular K + concentration of ATCC 29212, Δ mptD and + mptD . (c) Intracellular pH (pH in ) of ATCC 29212, Δ mptD and + mptD . (d) Cellular ATP concentration of ATCC 29212, Δ mptD and + mptD . * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: We compared the alkaline resistance of these six strains, along with two standard laboratory E. faecalis strains (ATCC 29212 and ATCC 51299), through alkaline resistance analysis.

Techniques: Concentration Assay, Fluorescence

Journal: Journal of Oral Microbiology

Article Title: The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis

doi: 10.1080/20002297.2025.2487944

Figure Lengend Snippet: The alkaline resistance evaluation of E. faecalis . (a) Dynamic growth curves of E. faecalis at pH 7 and 10. (b) Representative images of CFUs and CFUs-counting comparison among groups after incubation at pH 10 for 24 h. **** p < 0.0001.

Article Snippet: We compared the alkaline resistance of these six strains, along with two standard laboratory E. faecalis strains (ATCC 29212 and ATCC 51299), through alkaline resistance analysis.

Techniques: Comparison, Incubation

Journal: Journal of Oral Microbiology

Article Title: The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis

doi: 10.1080/20002297.2025.2487944

Figure Lengend Snippet: The number of differentially expressed genes of E. faecalis with different alkaline resistance under alkaline condition.

Article Snippet: We compared the alkaline resistance of these six strains, along with two standard laboratory E. faecalis strains (ATCC 29212 and ATCC 51299), through alkaline resistance analysis.

Techniques: Control, Modification, Transduction

Journal: Journal of Oral Microbiology

Article Title: The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis

doi: 10.1080/20002297.2025.2487944

Figure Lengend Snippet: Alkaline resistance of E. faecalis positively correlated with the expression of Man-PTS EII, membrane transport and amino acid metabolism genes. (a) KEGG enrichment analysis of upregulated or downregulated DEGs. (b) Selected differential expression genes involved in Man-PTS EII and membrane transport and amino acid metabolism. (c) Comparison of mRNA expression levels of Man-PTS EII at pH 10 by RT-qPCR in E. faecalis compared to pH 7. *** p < 0.001; ** p < 0.01.

Article Snippet: We compared the alkaline resistance of these six strains, along with two standard laboratory E. faecalis strains (ATCC 29212 and ATCC 51299), through alkaline resistance analysis.

Techniques: Expressing, Membrane, Quantitative Proteomics, Comparison, Quantitative RT-PCR

Journal: Journal of Oral Microbiology

Article Title: The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis

doi: 10.1080/20002297.2025.2487944

Figure Lengend Snippet: Illustration of potential role of Man-PTS EII in the alkaline resistance of E. faecalis .

Article Snippet: We compared the alkaline resistance of these six strains, along with two standard laboratory E. faecalis strains (ATCC 29212 and ATCC 51299), through alkaline resistance analysis.

Techniques:

Journal: MicrobiologyOpen

Article Title: Confocal laser scanning, scanning electron, and transmission electron microscopy investigation of Enterococcus faecalis biofilm degradation using passive and active sodium hypochlorite irrigation within a simulated root canal model

doi: 10.1002/mbo3.455

Figure Lengend Snippet: Mean values of surface area (μ 2 ) of E. faecalis biofilm on the canal surface at 3, 2, and 1 mm from the canal terminus, before and after irrigation protocols. The black arrow on the y ‐axis indicates breaks of different value axis scaling. Error bars are standard deviation ( n = 3 per group)

Article Snippet: Biofilms were grown from Enterococcus faecalis strain ( ATCC 19433), which was plated onto a BHI agar (Sigma‐Aldrich, St. Louis, Montana, USA) with 5% defibrinated horse blood and incubated at 37°C in the 5% CO 2 incubator for 24 hr.

Techniques: Standard Deviation

Journal: MicrobiologyOpen

Article Title: Confocal laser scanning, scanning electron, and transmission electron microscopy investigation of Enterococcus faecalis biofilm degradation using passive and active sodium hypochlorite irrigation within a simulated root canal model

doi: 10.1002/mbo3.455

Figure Lengend Snippet: CLSM (×20 magnification) images (0.3 mm 2 ) from within the root canal to illustrate (a) E. faecalis biofilm grown for 10 days and stained using Live/Dead ® viability stain with the green color indicating live cells and the red color showing the dead bacteria (control). (ai) residual biofilm at 3 mm from the canal terminus after syringe irrigation protocol. (b) Passive irrigation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus. (c) manual‐agitation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus. (d) Sonic agitation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus. (e) Ultrasonic agitation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus

Article Snippet: Biofilms were grown from Enterococcus faecalis strain ( ATCC 19433), which was plated onto a BHI agar (Sigma‐Aldrich, St. Louis, Montana, USA) with 5% defibrinated horse blood and incubated at 37°C in the 5% CO 2 incubator for 24 hr.

Techniques: Staining, Bacteria, Control

Journal: MicrobiologyOpen

Article Title: Confocal laser scanning, scanning electron, and transmission electron microscopy investigation of Enterococcus faecalis biofilm degradation using passive and active sodium hypochlorite irrigation within a simulated root canal model

doi: 10.1002/mbo3.455

Figure Lengend Snippet: SEM images (×2,000, ×8,000 magnification) illustrate (a) E. faecalis biofilm grown for 10 days onto the surface of the root canal model (control). (ai) residual biofilm at 3 mm from the canal terminus after syringe irrigation protocol. (b) Passive irrigation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus. (c) manual‐agitation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus. (d) Sonic agitation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus. (e) Ultrasonic agitation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus

Article Snippet: Biofilms were grown from Enterococcus faecalis strain ( ATCC 19433), which was plated onto a BHI agar (Sigma‐Aldrich, St. Louis, Montana, USA) with 5% defibrinated horse blood and incubated at 37°C in the 5% CO 2 incubator for 24 hr.

Techniques: Control

Journal: MicrobiologyOpen

Article Title: Confocal laser scanning, scanning electron, and transmission electron microscopy investigation of Enterococcus faecalis biofilm degradation using passive and active sodium hypochlorite irrigation within a simulated root canal model

doi: 10.1002/mbo3.455

Figure Lengend Snippet: TEM (×7,100, 31,000) images illustrate (a) E. faecalis biofilm grown for 10 days onto the surface of the root canal model (control). (ai) residual biofilm at 3 mm from the canal terminus after syringe irrigation protocol. (b) Passive irrigation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus. (c) manual‐agitation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus. (d) Sonic agitation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus. (e) Ultrasonic agitation group; (i) residual biofilm at 2 mm from the canal terminus; (ii) residual biofilm at 1 mm from the canal terminus

Article Snippet: Biofilms were grown from Enterococcus faecalis strain ( ATCC 19433), which was plated onto a BHI agar (Sigma‐Aldrich, St. Louis, Montana, USA) with 5% defibrinated horse blood and incubated at 37°C in the 5% CO 2 incubator for 24 hr.

Techniques: Control